Abstract
The tomato yellow leaf curl virus (TYLCV) was purified from infected tomato (Lycopersicon esculentum) and datura (Datura stramonium) plants. Typical twinned particles, characteristic of members of the geminivirus group, were detected in partially purified preparations obtained from TYLCV-infected source plants. Virus-containing fractions were infective as confirmed by membrane transmission test with the whitefly vector Bemisia tabaci. A circular single-stranded DNA containing about 2,800 nucleotides was isolated from the infective virus preparation.