Isolation of DNA from archival Papanicolaou stained cytological smears using a simple salting-out procedure
Open Access
- 1 February 1995
- journal article
- research article
- Published by BMJ in Molecular Pathology
- Vol. 48 (1) , M55-M56
- https://doi.org/10.1136/mp.48.1.m55
Abstract
DNA from archival Papanicolaou stained and unstained cytological smears was successfully isolated using a simple, rapid and inexpensive salting-out procedure. The quality of DNA was controlled by polymerase chain reaction (PCR) amplification of segments of the human β-globin, human β-actin and human papillomavirus L1 genes. Only negligible differences in amplification efficiency were observed between DNA isolated from stained and unstained smears. The salting-out procedure is a more rapid method for the isolation of DNA than phenol-chloroform extraction and may be used in instances where fresh or cryopreserved clinical specimens are not available.This publication has 6 references indexed in Scilit:
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