Microarray analysis reveals similarity between CD8+CD28− T cells from young and elderly persons, but not of CD8+CD28+ T cells
- 27 August 2008
- journal article
- research article
- Published by Springer Nature in Biogerontology
- Vol. 10 (2) , 191-202
- https://doi.org/10.1007/s10522-008-9167-1
Abstract
We isolated highly purified CD8+CD28+ and CD8+CD28− T cell populations from healthy young and elderly persons for gene expression profiling using Affymetrix oligonucleotide microarrays. We demonstrate that the gene expression profile of CD8+CD28− T cells is very similar in young and elderly persons. In contrast, CD8+CD28+ in elderly differ from CD8+CD28+ in young persons. Hierarchical clustering revealed that CD8+CD28+ in elderly are located between CD8+CD28+ in young and CD8+CD28− (young and old) T cells regarding their differentiation state. Our study demonstrates a dichotomy of gene expression levels between CD8+CD28+ T cells in young and elderly persons but a similarity between CD8+CD28− T cells in young and elderly persons. As CD8+CD28+ T cells from elderly and young persons are distinct due to a different composition of the population, these results suggest that the gene expression profile does not depend on chronological age but depends on the differentiation state of the individual cell types.Keywords
This publication has 27 references indexed in Scilit:
- PD-1 expression on HIV-specific T cells is associated with T-cell exhaustion and disease progressionNature, 2006
- CARMAweb: comprehensive R- and bioconductor-based web service for microarray data analysisNucleic Acids Research, 2006
- Serialin vivo loss andin vitro gain of Fas expression and function in human cancerous pancreatic duct cellsInternational Journal of Cancer, 2005
- The PANTHER database of protein families, subfamilies, functions and pathwaysNucleic Acids Research, 2004
- Aberrant Expression of the Tyrosine Kinase Receptor EphA4 and the Transcription Factor Twist in Sézary Syndrome Identified by Gene Expression AnalysisCancer Research, 2004
- Two subsets of memory T lymphocytes with distinct homing potentials and effector functionsNature, 1999
- The Complexities of T‐Cell Co‐stimulation: CD28 and BeyondImmunological Reviews, 1996
- Expansion of cytotoxic CD8+ CD28− T cells in healthy ageing people, including centenariansImmunology, 1996
- Thymic involution with ageing: obsolescence or good housekeeping?Immunology Today, 1996
- Reference Intervals for Human Peripheral Blood Lymphocyte Subpopulations from ‘Healthy’ Young and Aged SubjectsInternational Archives of Allergy and Immunology, 1995