A DNA-Binding Protein Specific for the Early Replicated Region of the Chromosome Obtained from Escherichia coli Membrane Fractions

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Abstract
After extensive dialysis of E. coli membranes treated with Triton X-100, 3 membrane proteins (A, B and B'') with an affinity for DNA were isolated and purified. They bind to double-stranded or single-stranded DNA. A and B'' proteins preferentially attach to DNA even in the presence of poly(uridylic acid). Only protein B'' can recognize some base sequence of DNA because pulse-labeled DNA made at the initiation of replication in a synchronized dnaC mutant was selectively retained by the protein.