A practicable variant of the ion exchange method for the radiometric estimation of ornithine decarboxylase activity
- 1 February 1987
- journal article
- research article
- Published by Springer Nature in Cellular and Molecular Life Sciences
- Vol. 43 (2) , 176-178
- https://doi.org/10.1007/bf01942841
Abstract
A known ornithine decarboxylase assay working with ion exchang separation of [3H]ornithine and [3H]putrescine has been revised. The assay can be performed in disposable 1.5 ml vessels with a total of four pipetting steps. The separation of enzyme substrate and product, respectively, requires 3 h per 50 samples. The detection limit is about 50 pmoles [3H]putrescine formed.Keywords
This publication has 13 references indexed in Scilit:
- Regulation of brain ornithine decarboxylase activity in the neonatal ratEuropean Journal of Pharmacology, 1983
- Developmental changes in mouse brain polyamine metabolismNeurochemical Research, 1982
- Ornithine decarboxylase (EC 4.1.1.17) assay based upon the retention of putrescine by a strong cation-exchange paperAnalytical Biochemistry, 1981
- A radiometric microassay for ornithine decarboxylaseNeuroscience, 1978
- Ornithine decar☐ylase assay using ion-exchange paperAnalytical Biochemistry, 1976
- Pyridoxal 5′‐Phosphate and the Regulation of Ornithine Decarboxylase Activity and StabilityEuropean Journal of Biochemistry, 1976
- Apparent kinetic differences between brain and liver ornithine decarboxylaseLife Sciences, 1976
- ORNITHINE DECARBOXYLASE ACTIVITY IN DEVELOPING RAT BRAIN1Journal of Neurochemistry, 1972
- A biosynthetic ornithine decarboxylase in Escherichia, coliBiochemical and Biophysical Research Communications, 1965
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934