THE PROPERTIES OF SONIC FRAGMENTS OF DEOXYRIBOSE NUCLEIC ACID

Abstract
By exposing DNA solutions to 9-kilocycle sonic waves for varying times in the absence of oxygen, the molecules were degraded through double-chain scission, producing fragments having the base-paired helical structure intact. In this manner, homologous samples of DNA were produced that covered a 25-fold range in molecular weight. With these fragments, the molecular-weight dependence of the sedimentation constant, the intrinsic viscosity, and the radius of gyration were determined.

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