ROLE OF FACTOR-XIII IN FIBROBLAST PROLIFERATION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 48  (2) , 135-142
Abstract
Fibrin stabilizing factor, factor XIII (F-XIII), which is a transamidating enzyme for fibrin crosslinking, is required for wound healing or fibroblast proliferation. To determine if the enzyme reacts directly with the cell surface, the [mouse embryonic] 3T6 fibroblasts were cultured on fibrin, which is a natural matrix for proliferation. Poor-crosslinked and crosslinked fibrin plates were prepared by human plasma clotting with or without the F-XIII inhibitors. In 48 h culture the seeded 3 .times. 104 cells were grown to 10.2 .times. 104 on the crosslinked fibrin but only to 3.8 .times. 104 on the poor-crosslinked fibrin plate on the average. The effect of an antifibrinolytic agent, .epsilon.-aminocaproic acid was also checked. The crosslinking of fibrin seemed to be essential to provide it with the favorable property promoting growth.