Cloning of the regulatory locus ompB of Salmonella typhimurium LT-2
- 1 December 1982
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 188 (2) , 184-189
- https://doi.org/10.1007/bf00332673
Abstract
We have cloned the complete functional ompB locus of Salmonella typhimurium LT-2 into Escherichia coli K-12 using a cosmid vector and in vitro packaging into λ. The ompB locus of Salmonella was found to complement both envZ and ompR mutations in E. coli as well as an ompR mutation of Salmonella. The ompR part of the ompB locus was further subcloned into the multicopy plasmid pKN410 as a 1.3 kb fragment. This fragment coded for a single 28.5 kd protein corresponding to about 820 bp in length. Furthermore, the OmpR proteins of S. typhimurium and E. coli were shown to be structurally and functionally highly similar.Keywords
This publication has 35 references indexed in Scilit:
- A rapid boiling method for the preparation of bacterial plasmidsPublished by Elsevier ,2004
- GENETIC ANALYSIS OF THE MAJOR OUTER MEMBRANE PROTEINS OF ESCHERICHIA COLIAnnual Review of Genetics, 1981
- Genetic analysis of the ompB locus in Escherichia coli K-12Journal of Molecular Biology, 1981
- The ompB locus and the regulation of the major outer membrane porin proteins of Escherichia coli K12Journal of Molecular Biology, 1981
- A small cosmid for efficient cloning of large DNA fragmentsGene, 1980
- Plasmids useable as gene-cloning vectors in an in vitro packaging by coliphage λ: “cosmids”Gene, 1978
- Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and MuJournal of Molecular Biology, 1976
- Nature of Col E 1 Plasmid Replication in Escherichia coli in the Presence of ChloramphenicolJournal of Bacteriology, 1972
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970