Persistent Infection of Human Lymphoid Cells with Poliovirus and Development of Temperature-Sensitive Mutants

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Abstract
Exposure of several spontaneously transformed human lymphoid cell lines to attenuated and virulent types 1 and 2 poliovirus strains led to an initial sharp increase in virus titer followed by a persistent infection. Virus was present as late as 80 passages after infection. The morphology, cell count and viability of virus- and mock-infected cells were similar. Approximately 1% of the cells scored as infectious centers and the cultures could be cured by specific antiserum. Virus produced during persistent infections initiated with a virus that readily forms plaques at 40.degree. C, Mahoney virus, showed an increase in temperature sensitivity and a decrease in plaque size.

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