DISCOVERY OF A NEW ACETATE-REPLACING FACTOR

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Abstract
Evidence is presented for the existence of a new factor required in the absence of acetate for the growth of a number of lactobacilli including Lactobacil-lus acidophilus (ATCC 4963), L. bifidus, L. bulgaricus strain 09 (ATCC 799 and 7993), and Thermobacterium acidophilus strain R26 (ATCC 11506). Commercial whey, fish solubles, distillers'' solubles, and certain yeast extracts are relatively rich sources of the factor. Details of a microbiological method for determination of the factor are described and procedure by which useful oncentrates of the factor may be obtained has been outlined.