Dendritic cells generated from patients with androgen‐independent prostate cancer are not impaired in migration and T‐cell stimulation

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Abstract
BACKGROUND: Dendritic cell (DC)‐based vaccination has been investigated as immunotherapy for several types of cancer. A potential drawback to vaccination with autologous monocyte‐derived DCs (MoDCs) could be that MoDCs from patients are functionally impaired. In case of androgen‐independent prostate cancer (CaP), the cancer itself, diverse prior therapies, and the hormone manipulation may affect the immune system.METHODS: MoDCs from patients suffering from androgen‐independent CaP were generated according to a clinically applicable protocol to evaluate the phenotype, maturation capacity, migration, and T‐cell stimulation of these cells compared with those generated from tumor‐free donors.RESULTS: MoDCs generated from CaP patients could be fully matured and efficiently migrated towards the chemokine CCL21. They had a strong potency to activate allogeneic CD4+ and CD8+ T‐cells and to present antigens to specific CTL.CONCLUSIONS: Our data suggest that MoDCs from patients with androgen‐independent CaP are functionally intact and hence qualify as cellular vaccines for immunotherapy of advanced stage CaP.

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