Influence of pH on capillary filtration coefficient of rat mesenteries perfused with solutions containing albumin.

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Abstract
A preparation of rat mesentery was vascularly isolated from the intestine and perfused with a physiological salt solution containing either Ficoll 70 or bovine serum albumin, to act as colloidal agents. The capillary filtration coefficient (Kf; U, ml min-1 100 g-1 mm Hg-1) was measured by following the weight change after graded increases in venous pressure. At pH values > 7.05, Kf, during perfusion with 3 and 4% bovine serum albumin solutions, was 0.219 .+-. 0.023 (mean .+-. [standard error of the mean]), 98 observations in 13 experiments, which was significantly < the value of 0.507 .+-. 0.038 which was obtained during perfusion with albumin solutions at pH < 7.05, 76 observations in 11 experiments, (P < 0.05). The value of Kf obtained during perfusion with 4% Ficoll solutions was 0.267 .+-. 0.018, 119 observations in 16 experiments, and remained uninfluenced by pH over the same range that was used with the albumin solutions; however, perfusion of the tissues with Ficoll solutions at pH > 7.05, after perfusion with albumin solution pH < 7.05, did cause the Ficoll-derived value of Kf to rise to 0.502 .+-. 0.055, 72 observations in 11 experiments. Evidently, the changes in Kf were not due to pH alone, but were mediated by albumin at acidic pH.