An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain
- 1 December 1982
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 39 (6) , 1751-1754
- https://doi.org/10.1111/j.1471-4159.1982.tb08015.x
Abstract
A modified filtration method for in vitro receptor binding was used to determine specific binding of [3H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 micrograms of protein per assay tube. Large differences in [3H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.Keywords
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