Defined Dimensional Changes in Enzyme Cofactors: Fluorescent "Stretched-Out" Analogs of Adenine Nucleotides
- 21 January 1977
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 195 (4275) , 296-298
- https://doi.org/10.1126/science.188137
Abstract
A concept is presented for testing the dimensional restrictions of enzyme-active sites by stretching the substrate or cofactor by known magnitude. These restrictions of enzyme-active sites specific for purine cofactors were tested by the synthesis and evaluation of lin-benzoadenosine 5'-triphosphate, 5'-diphosphate, and 3',5'-monophosphate with respect to enzyme binding and activity. These "stretchedout" (by 2.4 angstroms) versions of the adenine ribonucleotides bind strongly, slow the enzymatic rates, and have useful fluorescence properties.This publication has 18 references indexed in Scilit:
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