Mutational analysis of candidate genes in psychiatric disorders
- 15 December 1993
- journal article
- review article
- Published by Wiley in American Journal of Medical Genetics
- Vol. 48 (4) , 184-191
- https://doi.org/10.1002/ajmg.1320480404
Abstract
A genetic hypothesis for a disease presupposes the existence of variation in the DNA sequences of affected individuals. A series of techniques known together as “mutational analysis” can be applied towards identifying new sequence variations in selected genes. These techniques can screen a large series of individuals for mutations efficiently, so it is not necessary to determine the nucleotide sequence in every DNA sample. DNA samples suspected of harboring sequence variants are then sequenced. Denaturing gradient gel electrophoresis techniques, single stranded conformation polymorphism paradigms, and chemical cleavage of mismatches are 3 procedures widely used for the molecular screening of mutations today. We discuss each of these techniques for mutation screening.Keywords
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