The metabolism of specifically labelled glucose in bovine ketosis

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Abstract
Specifically labelled [1-C14]glucose and [6-C14]-glucose were synthesized and injected intravenously into ketotic cows. The results were compared with those obtained previously with normal cows. The recovery of C14 in respiratory carbon dioxide, casein, albumin and citrate after [6-C14]-glucose injection was less during ketosis than in the corresponding recovery in normal cows. This observation indicates a decrease of C14 transfer from glucose to carbon dioxide, casein, albumin and citrate or dilution of C14 from glucose by a relative increase in incorporation of carbon atoms from other precursors. or both of these possibilities, during ketosis. The results after [1-C14]glucose injection support these findings. The recovery of C14 in lactose and glycerol was the same in ketotic and normal cows after [6-C14]glucose injection, but higher in ketotic cows after [1-C14]glucose injection. Comparison of C14 recovery in the different milk constituents after [1-C14]glucose and [6-C14]-glucose injection shows a decrease of participation of the hexose mono-phosphate oxidative pathway during ketosis. The decrease seems to be correlated with impaired lipogenesis in ketotic cows.