Solubilization by nitrilotriacetic acid (NTA) of genetically active Cr(VI) and Pb(II) from insoluble metal compounds

Abstract
The frequencies of sister chromatid exchanges (SCE) were significantly increased in cultured Chinese hamster cells by insoluble salts of Cr(VI) (PbCrO4) and Pb(II) (PbSO4). A further significant increase of this effect was produced when diluted suspensions of PbCrO4 (1–4 mg/l) and PbSO4 (10–40 mg/l) were preincubated with nitrilotriacetic acid (NTA) concentrations (0.25–1 mg/l) such as those that are possibly found in highly contaminated environmental situations. NTA enhanced the induction of SCE by PbCrO4 and PbSO4 even in the presence, in the preincubation solution and in the medium used to treat the cells, of Na+ and K+ concentrations largely exceeding (e.g., 102‐106 times) those of the genotoxic metals.