A Kinetic Examination of Penicillin Acylase Stability in Water-organic Solvent Systems at Different Temperatures
- 1 January 2002
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis and Biotransformation
- Vol. 20 (1) , 53-56
- https://doi.org/10.1080/10242420210158
Abstract
We have studied the thermal stability of penicillin acylase from Streptomyces lavendulae in water-organic solvent monophasic systems at the range of temperatures between 40 and 60°C. We found a linear correlation between the log u P value of the solvent and the activation free energy for denaturation ( j G d ) at all temperatures tested. Thermodynamic analysis of the results indicates that solvents with log u P h m 2.3 have protective effects, whereas solvents with log u P S m 1.8 are deleterious for penicillin acylase.Keywords
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