A Study of Methylated Polyadenylic Acid
- 3 March 1971
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 19 (1) , 135-142
- https://doi.org/10.1111/j.1432-1033.1971.tb01297.x
Abstract
Copolymers with different contents of adenine and N1‐methyl adenine residues have been studied, polyadenylic acid being methylated by dimethylsulfate. Thermal stability, circular dichroism and light scattering measurements are in favor of a two‐strand structure at neutral pH. Thermal stability of these products is much less sensitive to ionic strength than that of most polynucleotides, and in some cases independent of ionic strength. These copolymers interact or not with poly(U), depending on the percentage of N1‐methyl adenine residues. Copolymers with high contents of methyladenine interact with poly(A) at neutral pH. These copolymers do not bind DNA‐dependent RNA polymerase. Some results at acid pH are also reported.Keywords
This publication has 19 references indexed in Scilit:
- Role of RNA Polymerase Stimulating Factor on Chain InitiationEuropean Journal of Biochemistry, 1969
- Etude des effets de la methylation sur le comportement de l'acide desoxyribonucleiqueBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
- Studies of the temperature-dependent conformation and phase separation of polyriboadenylic acid solutions at neutral pHJournal of Molecular Biology, 1967
- The Methylation of Nucleic AcidsAnnual Review of Biochemistry, 1966
- Adenylate oligomers in single- and double-strand conformationJournal of Molecular Biology, 1966
- Effects of Some Chemical Mutagens and Carcinogens on Nucleic AcidsProgress in Nucleic Acid Research and Molecular Biology, 1966
- Effect of partial methylation on properties of polyadenylic acidBiochemical and Biophysical Research Communications, 1965
- The conversion of two‐stranded poly (A + U) to, three‐strand poly (A + 2U) and poly A by heatBiopolymers, 1964
- 112. The methylation of adenosine and adenylic acidJournal of the Chemical Society, 1960
- The purine and pyrimidine composition of deoxypentose nucleic acidsBiochemical Journal, 1951