Structural Studies of Mutant Glucocorticoid Receptor Transactivation Domains Establish a Link between Transactivation Activity in Vivo and α-Helix-Forming Potential in Vitro

Publisher Website
Google Scholar
Abstract
We have previously shown, using circular dichroism spectroscopy, that the tau 1 core peptide has alpha-helix-forming potential in vitro [Dahlman-Wright et al. (1995) Proc. Natl. Acad. Sci. U.S.A. 92, 1699-1703]. The tau 1 core peptide is a 58-amino acid peptide, constituting the core of the transactivation activity of the tau 1 major transactivation domain of the human glucocorticoid receptor [Dahlman-Wright et al. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 1619-1623]. Further structural studies of the peptide, using NMR spectroscopy, identified three segments with alpha-helical character. In this report we show that reduced protein expression or stability is not responsible for the reduced in vivo transactivation potential of tau 1 core peptides with proline substitutions in proposed alpha-helical regions. Rather, the reduced alpha-helix propensity of the corresponding purified peptides in vitro suggests that alpha-helices are involved in the molecular mechanism of glucocorticoid receptor mediated changes in gene activity.