Detection of DNA Strand Breaks in Single Cells Using Flow Cytometry
- 1 January 1984
- journal article
- research article
- Published by Taylor & Francis in International Journal of Radiation Biology
- Vol. 46 (5) , 521-527
- https://doi.org/10.1080/09553008414551731
Abstract
A preliminary method is reported of alkaline unwinding of DNA within single cells and quantitation of the single-stranded and double-stranded DNA with the fluorescent probe acridine orange. A suspension of alkali-treated cells is obtained and analysed by flow cytometry. An increase in the amount of single-stranded DNA is taken as an indication of strand breaks. An advantage of this method is that a large number of cells can be individually analysed for DNA strand breaks. A measurement of DNA content is also obtained, making it possible to discriminate between cells in various parts of the cell cycle.Keywords
This publication has 8 references indexed in Scilit:
- Rapid isolation of metaphase chromosomes containing high molecular weight DNA.The Journal of cell biology, 1979
- ESTIMATION OF DNA STRAND BREAKS IN SINGLE MAMMALIAN CELLSPublished by Elsevier ,1978
- Cytofluorometric studies on conformation of nucleic acids in situ. I. Restriction of acridine orange binding by chromatin proteins.Journal of Histochemistry & Cytochemistry, 1976
- Cytofluorometric studies on conformation of nucleic acids in situ. II. Denaturation of deoxyribonucleic acid.Journal of Histochemistry & Cytochemistry, 1976
- Thermal denaturation of DNA in situ as studied by acridine orange staining and automated cytofluorometryExperimental Cell Research, 1975
- RAPID, SIMULTANEOUS MEASUREMENT OF DNA, PROTEIN, AND CELL VOLUME IN SINGLE CELLS FROM LARGE MAMMALIAN CELL POPULATIONSThe Journal of cell biology, 1973