Selective binding of antibody against gizzard 10‐nm filaments to different cell types in myogenic cultures

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Abstract
Antibody against the intermediate‐sized filaments from gizzard smooth muscle was used to determine the presence or absence of reacting 10‐nm filaments in different cell types. The antibody against gizzard 10‐nm filaments reacted with filaments in cultured smooth muscle cells, skeletal myotubes and postmitotic skeletal myoblasts. It did not bind to the 10‐nm filaments present in replicating presumptive myoblasts and fibroblasts, or the 10‐nm filaments in spinal ganglion cells.