Francisella tularensis live vaccine strain: Proteomic analysis of membrane proteins enriched fraction
- 3 June 2005
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 5 (9) , 2460-2467
- https://doi.org/10.1002/pmic.200401213
Abstract
Proteome analysis of Gram-negative facultative intracellular pathogen Francisella tularensis (F. tularensis) live vaccine strain has been performed only on whole-cell extracts so far. This is the first study dealing with the analysis of the membrane subproteome of this microorganism. A fraction enriched in membrane proteins obtained by carbonate extraction was separated using two-dimensional electrophoresis and all visualized spots were identified by mass spectrometry. The reference map is the basis for further comparative analyses of virulent and non-virulent F. tularensis strains.Keywords
This publication has 23 references indexed in Scilit:
- Comparative proteome analysis of cellular proteins extracted from highly virulent Francisella tularensis ssp. tularensis and less virulent F. tularensis ssp. holarctica and F. tularensis ssp. mediaasiaticaProteomics, 2004
- Characterization of Low Abundant Membrane Proteins Using the Protein Sequence Tag TechnologyJournal of Proteome Research, 2004
- Francisella TularensisPublished by Wiley ,2004
- PSORT-B: improving protein subcellular localization prediction for Gram-negative bacteriaNucleic Acids Research, 2003
- Proteomic analysis of Pseudomonas aeruginosa grown under magnesium limitationJournal of the American Society for Mass Spectrometry, 2003
- Will the enigma of Francisella tularensis virulence soon be solved?Trends in Microbiology, 2003
- The application of mass spectrometry to membrane proteomicsNature Biotechnology, 2003
- TularemiaClinical Microbiology Reviews, 2002
- Tularemia as a Biological WeaponJAMA, 2001
- Two-Dimensional Electrophoresis of Membrane Proteins Using Immobilized pH GradientsAnalytical Biochemistry, 2000