Rapid Determination of Acitretin or Isotretinoin and Their Major Metabolites by High-Performance Liquid Chromatography

Abstract
A method is described for the quantitative analysis of acitretin and its isomer metabolite, or of isotretinoin and its principal metabolites, in human plasma. Following a simple extraction, the compounds are determined by reversed-phase high-performance liquid chromatography (HPLC) and detection at 350nm. This method was applied to plasma specimens collected from healthy volunteers and patients receiving single or multiple dose administrations of these compounds.