Spurious Amplification of a Plasmodium vivax Small-Subunit RNA Gene by Use of Primers Currently Used To Detect P. knowlesi
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- 1 December 2009
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (12) , 4173-4175
- https://doi.org/10.1128/jcm.00811-09
Abstract
The PCR primers commonly used to detect Plasmodium knowlesi infections in humans were found to cross-react stochastically with P. vivax genomic DNA. A nested primer set that targets one of the P. knowlesi small-subunit rRNA genes was validated for specificity and for sensitivity of detection of <10 parasite genomes.Keywords
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