Complete sequence of the gene encoding the largest subunit of RNA polymerase I of Trypanosoma brucei
- 22 May 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 249 (1) , 123-128
- https://doi.org/10.1016/0014-5793(89)80029-8
Abstract
We have set out to clone the trypanosomal gene encoding the largest subunit of RNA polymerase I. We screened a genomic library with a synthetic oligonucleotide probe encoding an eleven amino acid sequence motif, YNADFDGDEMN, which has been found in all eukaryotic RNA polymerase largest subunit genes analyzed so far. We isolated the Trp11 locus and determined the complete sequence of the gene encoded within this locus. The deduced amino acid sequence contains the highly conserved RNA polymerase domains as well as the previously identified RNA polymerase I‐specific hydrophilic insertions. Therefore, the gene most closely resembles the largest subunit of RNA polymerase I.Keywords
This publication has 25 references indexed in Scilit:
- Essential roles of the RNA polymerase I largest subunit and DNA topoisomerases in the formation of fission yeast nucleolus.The Journal of cell biology, 1989
- Trypanosoma brucei contains two RNA polymerase II largest subunit genes with an altered C-terminal domainCell, 1989
- Major nucleolar proteins shuttle between nucleus and cytoplasmCell, 1989
- Cloning and transcriptional analysis of a variant surface glycoprotein gene expression site in Trypanosoma bruceiMolecular and Biochemical Parasitology, 1988
- The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. bruceiCell, 1987
- VSG gene 118 is transcribed from a cotransposed pol I-like promoterCell, 1987
- Eukaryotic RNA polymerase I promoter binding is directed by protein contacts with transcription initiation factor and is DNA sequence-independentCell, 1987
- Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerasesCell, 1985
- Eukaryotic RNA PolymeraseCritical Reviews in Biochemistry, 1985
- Amatoxins, Phallotoxins, Phallolysin, and Antamanide: The Biologically Active Components of PoisonousAmanitaMushroomCRC Critical Reviews in Biochemistry, 1978