Induction of Antibodies in Rats to a Rat Carcinoembryonic Antigen

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Abstract
In a previous communication, carcinoembryonic antigen (CEA) of rat, which is an analogue to human CEA, was demonstrated in gastrointestinal adenocarcinomas induced in inbred Fischer rats by injection of 1,2-dimethylhydrazine. Antibodies detectable by enzyme immunoassay were elicited in Fischer rats by immunization with a perchloric acid extract of the CEA-containing rat tumor, RCA-1, incorporated into Freund’s complete adjuvant. Specificity studies showed that activity of the rat antisera could be virtually abolished by inhibition with the tumor extract used at a concentration of 25 μg/ml. Inhibition by newborn rat tissues required extract at a concentration of 250 μg/ml. Extracts of normal adult tissues did not inhibit at these concentrations, but did inhibit at a concentration of 2,500 μg/ml. The results showed that rat CEA, though present in low concentration in normal adult rat tissue, is capable of eliciting an immune response in rats.

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