Establishment of Isolated Root Cultures of Brassica Species and Regeneration from Cultured-root Segments of Brassica oleracea var. italica

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Abstract
Isolated root cultures which could be maintained over several months by serial subculture were established from Brassica oleracea var. italica cv. Green Comet F1. A modified White's medium was found to be the best of several salt compositions tested. The effects on isolated root growth of the following were also examined; nutritional components, culture vessel type and closure, pH of the medium and auxin type and concentration. Using a medium devised for Green Comet, root cultures were established from six other B. oleracea, B. napus and B. campestris cultivars. It was possible to regenerate shoots from segments of cultured roots by incubation on agar-solidified media containing cytokinin and auxin. The effects on regeneration of various auxins and cytokinins were investigated; the combination of Picloram with KN gave the highest frequency of shoot formation. It was demonstrated that isolated roots retained their regenerative ability over a period of 5 months in culture.