Group Experiments on the Radioimmunological Insulin Determination

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Abstract
By means of two group experiments, performed in seven laboratories and using six different modifications in the insulin radioimmunoassay technique, it is shown that the use of standards diluted in human serum of low basal insulin concentration, instead of buffer, improves appreciably the interlaboratory precision. The causes lies perhaps in some serum factor, which could disturb some modifications of the radioimmunoassay more than others. There was no improvement of precision when the same insulin standard was used in all laboratories. Standard curves made up in different human fasting sera have a scatter only within the range of pipetting and counting error. 1 Supported by the Deutsche Forschungsgemeinschaft (SFB 51, München, and SFB 87, Ulm).

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