EFFECTS OF TETRACYCLINE AND DIAZEPAM ON INSULIN-SECRETION AND ADENYLYL CYCLASE ACTIVITY OF ISOLATED RAT ISLETS OF LANGERHANS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 3  (1) , 3-6
Abstract
Tetracycline (2 and 20 .mu.M) inhibited glucose-stimulated insulin secretion from isolated rat islets of Langerhans, although it failed to alter tolbutamide-stimulated insulin release. Diazepam (10 .mu.M) potentiated tolbutamide-stimulated insulin release and at a concentration of 1 mM increased glucose-stimulated insulin release. Tetracycline (20 and 200 .mu.M) or diazepam (10, 100 and 1000 .mu.M) inhibited adenylate cyclase activity of the islets'' homogenate. The effect of tetracycline on insulin secretion might be in part due to inhibition of adenylate cyclase of the islets. The effect of diazepam on insulin secretion is not mediated through the adenylate cyclase system.

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