Cooperative Pathway for Lipid Biosynthesis in Young Pea Leaves: Oleate Exportation from Chloroplasts and Subsequent Integration into Complex Lipids of Added Microsomes

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Abstract
In vitro [1-14C]-acetate incorporation into pea chloroplast lipids resulted in the synthesis of palmitic and oleic acids. Separation of chloroplasts after the incorporation proved the specific exportation of [14C]-oleate towards the external medium. The addition of microsomes resulted in a stimulated exportation and integration of the exported oleate into the phosphatidylcholine (PC). In such experiments, the galactolipids were labelled by both palmitate and oleate and confirmed the intraplastidial origin of the fatty acids they incorporated. Isolated chloroplasts alone performed an acylation of PC by labelled oleate. The chloroplastic localization of this activity is discussed.