Semiautomated preparation of DNA templates for large-scale sequencing projects
- 1 January 1990
- journal article
- research article
- Published by Taylor & Francis in DNA Sequence
- Vol. 1 (1) , 73-78
- https://doi.org/10.3109/10425179009041349
Abstract
The rate limiting step in a large-scale sequencing project is the generation of single-stranded DNA templates. We describe a fast, semiautomated procedure, using 96-well microtitre plates, in which 192 templates can be readily prepared in 1 day. The technique can be carried out manually or can be semiautomated using a robot pipetting device. We also provide evidence for the reliability and applicability of this method to a large-scale sequencing project.Keywords
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