STUDIES ON POLYPEPTIDES OF POXVIRUS .2. COMPARISON OF VIRUS-INDUCED POLYPEPTIDES IN CELLS INFECTED WITH VACCINIA, COWPOX AND SHOPE FIBROMA VIRUSES

  • 1 January 1978
    • journal article
    • research article
    • Vol. 21  (3) , 77-94
Abstract
Virus-induced polypeptides in [rabbit kidney RK-13 cells] cells infected with vaccinia, cowpox and Shope fibroma viruses were examined by SDS[sodium dodecyl sulfate]-polyacrylamide gel electrophoresis followed by autoradiography. At least 42 vaccinia virus-induced polypeptides were identified among the polypeptides of cells pulse-labeled with [35S]-methionine and/or of fractionated cells labeled with [14C]-leucine for 24 h. They consisted of 15 polypeptides (early polypeptides) which were synthesized even in the presence of cytosine-1.beta.-D-arabinofuranosyl-HCl, and 27 polypeptides (late polypeptides) which were synthesized only in the absence of cytosine-1-.beta.-D-arabinofuranosyl-HCl. By the same procedure at least 40 cowpox virus-induced polypeptides (14 early polypeptides and 26 late polypeptides) and at least 31 Shope fibroma virus-induced polypeptides (13 early polypeptides and 18 late polypeptides) were identified. Comparative studies of virus-induced polypeptides on the basis of migration in SDS-polyacrylamide gel electrophoresis revealed that 11 polypeptides were early polypeptides common to both vaccinia and cowpox viruses; 21 were late polypeptides common to both vaccinia and cowpox viruses; 4 were early polypeptides common to both vaccinia and Shope fibroma viruses; 7 were late polypeptides common to both vaccinia and Shope fibroma viruses; 5 were early polypeptides common to both cowpox and Shope fibroma viruses; 9 were late polypeptides common to both cowpox and Shope fibroma viruses; 4 were early polypeptides common to all 3 viruses; and 7 were late polypeptides common to all 3 viruses.