Analysis of transcription and processing signals in the 5′ regions of the two Mycoplasma capricolum rRNA operons

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Abstract
The transcription and RNA processing signals of the rRNA operons (rrnA and rrnB) of Mycoplasma capricolum were analyzed by mapping the 5′ ends of in vivo and in vitro synthesized RNAs. The results of both in vitro and in vivo analyses point to the rrnA operon being transcribed from two promoters (P1 and P2) into large precursor RNAs. Transcripts initiating at P1 contain two tRNAs, and probably 16 S, 23 S, and 5 S rRNAs, whereas the transcripts starting from P2 consist only of the three rRNAs. The precursor RNAs are processed via distinct intermediates into mature tRNAs and rRNAs. In vivo experiments indicated that the rrnB operon is transcribed only from one promoter, although a second promoter could be identified using cell free extracts. The rrnB operon does not contain tRNA genes, but the precursor is still processed in the same way as the rrnA precursor that is synthesized from P2.