Production of a High Affinity Antibody Specific to the Calcium-Free-Form of Calmodulin, Using N-Acetyl-muramyl-L-alanyl-D-isoglutamine-calmodulin Conjugate
- 1 January 1983
- journal article
- research article
- Published by Taylor & Francis in Journal of Immunoassay
- Vol. 4 (4) , 395-406
- https://doi.org/10.1080/15321818308057017
Abstract
Covalent coupling of N-acetyl-muramyl-L-alanyl-D—isoglutamine (MDP) to calmodulin was used in order to enhance its immunogenicity. Rabbit antibodies against calmodulin were obtained, interacting with the calcium-free-form of calmodulin. The radioimmunoassay was developed using the whole sera (titre 1:20,000) having a sensitivity of 20 pg per tube (50% displacement 500 pg/ assay tube). The radioimmunoassay shows the same concentrations of calmodulin in rat brain homogenate (4.9 ± 0.86 μg/mg protein), bovine neurosecretosomes (0.77 ± 0.10 μg/mg protein), and bovine neurohypophysial secretory vesicles (0.05 ± 0.01 μg/mg protein) as the phosphodiesterase activation method.Keywords
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