Identification of B2 Bradykinin Binding Sites on Cultured Cortical Astrocytes
- 5 October 1991
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 57 (4) , 1456-1458
- https://doi.org/10.1111/j.1471-4159.1991.tb08314.x
Abstract
Bradykinin was found to bind to specific high-affinity sites in cultured cortical astrocytes from rat brain, and this binding appeared to be specific for the B2 bradykinin receptor subtype. Nonlinear regression analysis of saturation experiments using a computer programme revealed a single KD of 16.6 ± 2.6 nM and a Bmax of 352.2 ±30.7 fmol/mg of protein. These results indicate that astrocytes possess bradykinin receptors and that these are predominantly of the B2 subtype.Keywords
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