HAPTEN SPECIFIC IGE ANTIBODY-RESPONSES IN MICE .5. DIFFERENTIAL RESISTANCE OF IGE B-LYMPHOCYTES AND IGG B-LYMPHOCYTES TO X-IRRADIATION

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  • 1 January 1976
    • journal article
    • research article
    • Vol. 117  (5) , 1622-1628
Abstract
These studies were done to determine whether IgE[immunoglobulin E]- and IgG-producing murine B [bone marrow-derived] lymphocytes could be distinguished by differential resistance to X-irradiation in vivo. Sensitivity or resistance of B lymphocytes to various doses of X-irradiation was monitored by measuring the production by irradiated cells of hapten-specific IgG and IgE antibody. Primed and unprimed IgG B lymphocytes are less radioresistant than corresponding IgE B lymphocytes. Primed IgG B lymphocytes are more radioresistant than unprimed IgG B cells; unprimed IgE B lymphocytes are at least as radioresistant as primed IgE B lymphocytes. The relative radioresistance of primed IgE vs. IgG B cells was best demonstrated in an adoptive transfer system. Exposure of ASC[Ascaris suum extract]-primed mice to 300 R 24 h after transfer into these mice of syngeneic, DNP-KLH[dinitrophenylated-keyhole limpet hemocyanin]-primed spleen cells led to a sustained failure to produce IgG anti-DNP antibody after challenge with DNP-ASC; under these conditions, IgE anti-DNP production was not impaired. This reflected differential radioresistance of the corresponding B cell populations. Exposure to X-irradiation tended to augment IgE anti-DNP antibody responses when compared to unirradiated controls. This phenomenon probably reflects the effects of X-irradiation on regulatory mechanisms controlling the mouse IgE response. The results are discussed in the light of evidence suggesting that, in mouse and man, IgE B lymphocytes have certain unique characteristics by which they may be distinguished from other classes of B lymphocytes.

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