Oxidation reactions catalyzed by manganese peroxidase isoenzymes from Ceriporiopsis subvermispora
- 4 September 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 371 (2) , 132-136
- https://doi.org/10.1016/0014-5793(95)00874-9
Abstract
A total of 11 manganese peroxidase isoenzymes (MnP1-MnP11) with isoelectric points (pIs) in the range of 4.58–3.20 were isolated from liquid- and solid-state cultures of the basidiomycete Ceriporiopsis subvermispora. In the presence of hydrogen peroxide, these isoenzymes showed different requirements for Mn(II) in the oxidation of vanillylacetone, o-dianisidine, p-anisidine and ABTS, whereas oxidation of guaiacol by any isoenzyme did not take place when this metal was omitted. Km values for o-dianisidine and p-anisidine in the absence of Mn(II) are in the range of 0.5–1.0 mM and 4.5–42.0 mM, respectively. Oxalate and citrate, but not tartrate, accelerate the oxidation of o-dianisidine, both in the presence and in the absence of Mn(II). MnPs from this fungus are able to oxidize kojic acid without externally added hydrogen peroxide, indicating that they can also act as oxidases. In this reaction, however, the requirement for Mn(II) is absoluteKeywords
This publication has 28 references indexed in Scilit:
- Isoenzymes of manganese-dependent peroxidase and laccase produced by the lignin-degrading basidiomycete Ceriporiopsis subvermisporaMicrobiology, 1994
- Participation of Mn(II) in the catalysis of laccase, manganese peroxidase and lignin peroxidase from Phlebia radiataFEBS Letters, 1994
- Mechanism of Manganese Peroxidase Compound II Reduction. Effect of Organic Acid Chelators and pHBiochemistry, 1994
- Lignin-modifying enzymes from selected white-rot fungi: production and role from in lignin degradationFEMS Microbiology Reviews, 1994
- Manganese, Mn-dependent peroxidases, and the biodegradation of ligninBiochemical and Biophysical Research Communications, 1988
- Manganese peroxidase from the basidiomycete Phanerochaete chrysosporium: spectral characterization of the oxidized states and the catalytic cycleBiochemistry, 1988
- Enzymatic activities of an extracellular, manganese-dependent peroxidase fromPhanerochaete chrysosporiumFEMS Microbiology Letters, 1985
- Kojic acid, a tyrosinase inhibitor from Aspergillus albus.Agricultural and Biological Chemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970