Spectrophotometric determination of benzyl penicillin in pharmaceutical preparations using copper(II) acetate as a complexing agent

1 January 1986

journal article
Published by Royal Society of Chemistry (RSC) in The Analyst

Vol. 111 (10) , 1179-1181
https://doi.org/10.1039/an9861101179

Abstract

Copper(II) acetate is proposed as a chromogenic reagent for the spectrophotometric determination of benzyl penicillin. The reagent forms a green 2 : 1 complex with benzyl penicillin at pH 6–6.8. This complex is stable for nearly 25 min after its formation. The green species has an absorption maximum at 650 nm with a molar absorptivity of 0.24 × 10³ l mol^–1 cm^–1. Beer's law is valid over the concentration range 20–1000 µg ml^–1 of benzyl penicillin. The precision of the method is 1.89%. The reagent has been successfully used for the spectrophotometric determination of benzyl penicillin alone and in pharmaceutical preparations.

Keywords

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