15N/14N Analysis of Amino Acids with GC-C-IRMS - Methodical Investigations and Ecotoxicological Applications
- 1 December 1995
- journal article
- research article
- Published by Taylor & Francis in Isotopes in Environmental and Health Studies
- Vol. 31 (3-4) , 367-375
- https://doi.org/10.1080/10256019508036284
Abstract
In order to perform the 15N/14N analysis of amino acids using a gas chromatograph and isotope ratio mass spectrometer linked up via a combustion interface (GC-C-IRMS), the amino acids must be derivatized. Tert-butyldimethylsilylation is examined using various techniques (direct conversion to nitrogen gas, ConFlo-IRMS [1], GC-C-IRMS [2]) and subsequently applied to isotopic characterization of amino acids from wheat protein hydrolyzate obtained from plants exposed to ozone. The method provides a reliable tool for studying ecotoxicological effects on plants at a molecular level in addition to the investigation of the natural variations of different N fractions.Keywords
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