ANTIGENIC ANALYSIS OF HEMATOPOIESIS .2. EXPRESSION OF HUMAN NEUTROPHIL ANTIGENS ON NORMAL AND LEUKEMIC MARROW-CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 63  (3) , 574-578
Abstract
The binding of 5 antineutrophil monoclonal autobodies ANH-1, -2, -3, -7, and -8, to normal and leukemic bone marrow cells was studied. AHN-7 bound to many granulocytic precursors, particularly myelocytes, and both lymphoid and blast cells in normal marrow, and to most but not all granulocyte-macrophage progenitors (CFC-GM). AHN-8 bound only to late (band and segmented) neutrophilic cell and not to CFG-GM. AHN-1, -2, -3 bound to morphologically identifiable neutrophil precursors, but not to (day-14) CFC-GM. Approximately 1/2 of nonlymphoid leukemia specimens were positive with AHN-1 or AHN-7; by contrast, lymphoid leukemia specimens wre rarely positive. AHN-8 was rarely found in leukemic cells. These antineutrophil antibodies appear to detect distinct granulopoietic subsets and may be useful in the analysis of hematologic differentiation and in the subclassification of leukemias.

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