Changes in properties of phosphoenolpyruvate carboxylase from the CAM plant Sedum praealtum D.C. upon dark/light transition and their stabilization by glycerol

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Abstract
A prenounced decrease in phosphoenolpyruvate earboxylase (PEPC) activity is observed upon dark/light transition in Sedum praealtum D.C., only when glycerol is included in the extraction medium. If glycerol is omitted, the activity extracted in light is initially low, but soon reaches night levels. The stabilization of the light-induced form of the enzyme by glycerol, in crude or desalted extracts, made it possible to study its kinetic properties in comparison to those of the dark form. The behaviour towards substrate (PEP) changes from hyperbolic (dark) to sigmoid (light), S0.5 is increased and the enzymic activity becomes more sensitive to malate inhibition. Quite different activity/pH profiles are also obtained for the two forms of PEPC. It is inferred that the in vivo regulation of PEPC in CAM is effected by a concerted action of light, malate and pH shifting.