Determination of Nicotine in Serum by TLC-Densitometry
- 1 March 1992
- journal article
- pharmaceutical analysis
- Published by Taylor & Francis in Analytical Letters
- Vol. 25 (3) , 485-496
- https://doi.org/10.1080/00032719208016110
Abstract
A TLC-Densitometric procedure for determination of nicotine in serum is reported. Nicotine was extracted with ethylacetate from protein free serum almost quantitatively and evaporated to dryness with a stream of nitrogen. The residue was oxidized to nicotinic acid with concentrated nitric acid, diluted with methanol to a standard volume and then determined quantitatively by TLC-densitometor at λmax262 nm.Keywords
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