Molecular Comparison of Isolates of Burkholderia multivorans from Patients with Cystic Fibrosis in the United Kingdom
Open Access
- 1 December 2003
- journal article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (12) , 5750-5754
- https://doi.org/10.1128/jcm.41.12.5750-5754.2003
Abstract
Burkholderia multivorans strains from 47 cystic fibrosis (CF) patients in 28 hospitals were compared by pulsed-field gel electrophoresis (PFGE) and flagellin ( fliC ) PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. A considerable degree of genetic variation was evident, with each patient harboring a strain with a unique PFGE profile. Four sizes of fliC amplicons were produced, and these amplicons gave 13 RFLP types with restriction enzyme Msp I. B. multivorans did not appear to spread between patients, suggesting that most CF patients acquire the organism from the natural environment.Keywords
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