Human Amnion Cell Cultures; Susceptibility to Viruses and Use in Primary Virus Isolations.

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Abstract
Tissue cultures of human amnion cells were prepared by growing cells obtained from amniotic membranes by tryptic digestion in Eagle''s basal medium supplemented with 10% horse serum. The susceptibility of amnion cells to a number of viruses was investigated. Adenoviruses types 1 through 8, Coxsackie A9, B1, B3, and B5, and herpes simplex virus multiplied and produced cytopathic changes in amnion cultures. Coxsackie A1 through 8, influenza A and B, type 1 dengue, mumps, and Newcastle disease virus showed no evidence of multiplication. Amnion cell cultures were employed in large scale routine testing for viral agents from throat swabs and washings collected from persons with febrile respiratory illnesses. Forty strains of adenoviruses types 1, 2, 3, and 5, one type 1 poliovirus, and 6 strains of herpes simplex virus were isolated from these samples.