SIGNIFICANCE OF MITOCHONDRIAL ENHANCEMENT IN RESTORING HEPATIC ENERGY CHARGE AFTER REVASCULARIZATION OF ISOLATED ISCHEMIC LIVER

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Abstract
The critical point of reversibility in [canine] hepatic ischemia was investigated. Immediately after the liver was isolated, the hepatic energy charge decreased rapidly from the control level of 0.85 to 0.28 and remained at a constant value during 30-, 60- and 90-min ischemic periods. After revascularization of the liver, the rapidity toward the normalization of the energy charge and the mitochondrial phosphorylative activity were different according to the ischemic time. The livers after 30- and 60-min exclusion showed a normalization of the energy charge and the enhancement of mitochodnrial phosphorylative activity, while the livers after 90-min exclusion showed only a slight increase of energy charge, which remained far from the normal value, and the enhancement of mitochondrial phosphorylative activity was not observed. A rapid enhancement in phosphorylative activity of the liver mitochondria after revascularization may play a decisive role on the restoration of the viability of isolated ischemic liver.

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