Discrete Determinants in Transfer RNA for Editing and Aminoacylation
- 23 May 1997
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 276 (5316) , 1250-1252
- https://doi.org/10.1126/science.276.5316.1250
Abstract
During translation errors of aminoacylation are corrected in editing reactions which ensure that an amino acid is stably attached to its corresponding transfer RNA (tRNA). Previous studies have not shown whether the tRNA nucleotides needed for effecting translational editing are the same as or distinct from those required for aminoacylation, but several considerations have suggested that they are the same. Here, designed tRNAs that are highly active for aminoacylation but are not active in translational editing are presented. The editing reaction can be controlled by manipulation of nucleotides at the corner of the L-shaped tRNA. In contrast, these manipulations do not affect aminoacylation. These results demonstrate the segregation of nucleotide determinants for the editing and aminoacylation functions of tRNA.Keywords
This publication has 14 references indexed in Scilit:
- Aminoacylation error correctionNature, 1996
- Residues in a Class I tRNA Synthetase Which Determine Selectivity of Amino Acid Recognition in the Context of tRNABiochemistry, 1995
- Mutational Isolation of a Sieve for Editing in a Transfer RNA SynthetaseScience, 1994
- Molecular Recognition of the Identity-determinant Set of Isoleucine Transfer RNA from Escherichia coliJournal of Molecular Biology, 1994
- The Transfer RNA Identity Problem: a Search for RulesScience, 1994
- Recognition of †RNAs by Aminoacyl-†RNA SynthetasesProgress in Nucleic Acid Research and Molecular Biology, 1991
- Codon and amino-acid specificities of a transfer RNA are both converted by a single post-transcriptional modificationNature, 1988
- Evidence for the double-sieve editing mechanism in protein synthesis. Steric exclusion of isoleucine by valyl-tRNA synthetasesBiochemistry, 1979
- Three-Dimensional Tertiary Structure of Yeast Phenylalanine Transfer RNAScience, 1974
- Structure of yeast phenylalanine tRNA at 3 Å resolutionNature, 1974