Characterization of the Collagen Synthesized by Cultured Human Smooth Muscle Cells from Fetal and Adult Aorta
- 1 January 1977
- journal article
- research article
- Published by Walter de Gruyter GmbH in Hoppe-Seyler´s Zeitschrift Für Physiologische Chemie
- Vol. 358 (1) , 401-408
- https://doi.org/10.1515/bchm2.1977.358.1.401
Abstract
Smooth muscle cells were grown from explants of the tunica media of fetal and adult human aorta. Collagen was isolated after incubation with 14C-glycine and was characterized by ion exchange chromatography. All cells investigated synthesized 2 types of collagen: type I (chain composition [.alpha.1(I)]2.alpha.2) and type III (chain composition [.alpha.1(III)]3). The collagen made by cells from adult donors contained approximately 70% type I and 30% type III collagen. This corresponded to the collagen composition in the original tissue. No age-related change in the type I/type III ratio was found with cells from donors between 9-67 yr of age. The type III portion of the collagen made by fetal cells was markedly less (about 15-20% of total collagen).This publication has 2 references indexed in Scilit:
- Synthesis of collagens Types I and III by pig medial smooth muscle cells in cultureBiochemical and Biophysical Research Communications, 1976