Impaired Processing of Ribosomal Precursor RNA in Blast Cells of Acute Leukemia
- 1 January 1971
- journal article
- rna metabolism
- Published by S. Karger AG in Acta Haematologica
- Vol. 45 (3) , 201-208
- https://doi.org/10.1159/000208625
Abstract
Homogeneous blast cell populations from 9 cases of acute leukemia were incubated for periods varying from 30 min to 6 h simultaneously with uridine-5–3H and 14C-methyl-methíonine. The distribution of label in the different classes of RNA molecules was studied after their separation by sedimentation in sucrose gradients. At variance with normal proliferating blood cells, in leukemic blasts, methylation and cleavage of 45 S precursor RNA occurred at a very low rate. Furthermore, an accumulation of unmethylated labeled RNA molecules in the size range of ribosomal precursor RNA was observed, presumably due to an unbalance between rate of synthesis and rate of processing of 45 S RNAKeywords
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