Recovery of Enterovirus from Primary Sludge Using Three Elution Concentration Procedures

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Abstract
Recovery of virus from sewage sludge is usually performed using an elution of adsorbed virus with alkaline pH solution followed by a concentration step before inoculation on cell cultures. In the present study three extraction-concentration procedures have been compared testing 40 g of solid fraction sludge. The highest virus recovery is obtained using an elution with 3 % beef extract - 0.1 M borate buffer pH 9 associated to a polyethyleneglycol precipitation as concentration step. Heterogeneous and lower results are obtained when the elution step uses a 1 % skim milk solution pH 9 or a glycin 0.05 M solution pH 9 and an organic flocculation as concentration step.

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